Plasmid_Backbone

Part:BBa_K3789008:Design

Designed by: MEI YING YI   Group: iGEM21_UM_Macau   (2021-09-26)


pFA6a


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1852
    Illegal SpeI site found at 1876
    Illegal PstI site found at 29
    Illegal PstI site found at 616
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1852
    Illegal SpeI site found at 1876
    Illegal PstI site found at 29
    Illegal PstI site found at 616
    Illegal NotI site found at 3
    Illegal NotI site found at 1889
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1852
    Illegal BglII site found at 406
    Illegal BamHI site found at 41
    Illegal BamHI site found at 124
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1852
    Illegal SpeI site found at 1876
    Illegal PstI site found at 29
    Illegal PstI site found at 616
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1852
    Illegal SpeI site found at 1876
    Illegal PstI site found at 29
    Illegal PstI site found at 616
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 1906
    Illegal BsaI.rc site found at 3087
    Illegal SapI site found at 2004


Design Notes

The pFA6a plasmid is a commonly used backbone plasmid for generating epitope tagging vectors. Our team members need to use pFA6a because it contains the HA tag, KanMX, and ADH1 terminator. Besides, it is also specially designed for yeast expression.



Source

Company synthesis

References